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In PCOS patients, hyperphosphorylation of serine residues ( e.g ., Ser307, Ser636) on IRS-1/2 suppresses their tyrosine phosphorylation, thereby impairing the downstream PI3K/AKT signaling pathway

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Subsequently, slides were rinsed in distilled water and then counterstained with nuclear-fast red for 5 min at room temperature

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