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The tested edge activators were added to distilled water (10 ml) and heated at the same temperature (70 C) to develop an aqueous phase

Once the packaging cells are transfected and infected, they will produce infectious AAV viral particles which contain the polynucleotide construct of the invention

Cells were washed three times with PBS, then lysed with 200 l of 0.1% Triton X-100 for 5 min

The various liposome-nucleic acid complexes are prepared using methods well known in the art

In conclusion, for any oxidative stress states such as cold and flu infections, there is nothing more effective than a high abosrbency antioxidant supplement to reduce the severity of an infection

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