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For trimix, due to the delicate nature of alprostadil the beyond use date for an unpunctured vial is 30 days

This makes it the preferred choice for research applications prioritizing maximum efficacy, controlled conditions, and individuals comfortable with injection protocols

10.1038/nrg3868 122 RojasL

For instance, a 500 mcg single daily dose is ~0.3 mL (~30 units)

TNF-mediated necroptosis in brain endothelial cells as a potential mechanism of increased seizure susceptibility in mice following systemic inflammation

Still, its important to keep in mind that this study was very small, and what works for some people may not work for others