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2.5.1.1 Small molecules analysis Compounds in the samples analyzed between 2020 and 2023 were identified using a high-performance liquid chromatograph (Ultimate 3,000 system from Dionex, Thermo Fisher Scientific, Waltham, MA, United States) coupled with a high-resolution micrOTOF-QII hybrid mass spectrometer (Bruker Daltonik, Bremen, Germany)

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Research subjects typically administer 0.1 to 0.3 mg via subcutaneous injection 30 to 60 minutes before bedtime, with cycles running 4 to 8 weeks followed by a break period