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2006;290:H295303

PMID 17717316.

Mesenchymal stem cells: Mechanisms of inflammation

Shen C, Tannenbaum D, Horn R, Rogers J, Eng C, Zhou S, Johnson B, Kopetz S , Morris V, Overman M, Parseghian C, Chang GJ, Lopez-Olivo MA, Kanwal R, Ellis LM, Dasari A

Briefly, cells pretreated with 4 mM glutamine (Gln) and 0 mM Gln, respectively, were cross-linked with formaldehyde at 37 C for 10 min, followed by the addition of glycine solution to quench the cross-linking reaction

Cancer cells often rely on pathways that are under tight regulation in healthy tissues, including the de novo cholesterol, lipid, and nucleotide synthesis pathways [25, 44, 45] to increase tumour mass and sustain high proliferation [46]