For alternative elution buffers, please refer to this Tech Tip( Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
doi:10.1159/000097552
Patients who supplement B12 during tirzepatide therapy commonly report better sustained energy levels compared to those who do not
Regular exercise increases metabolic rate, generates body heat, and helps preserve lean muscle mass during weight loss
Abbreviations PTMs: Post-translational modifications PATs: Protein acyltransferases DHHC: Asp-His-His-Cys zDHHC: Zinc finger DHHC KAT/KDAC: Lysine acetyltransferase/lysine deacetylase NATs: N-terminal acetyltransferases SIRT3: Sirtuin 3 MAPK: Mitogen-activated protein kinase MKP1: MAPK phosphatase 1 MDM2: Mouse double minute 2 homolog KDAC: Lysine deacetylase TSA: Trichostatin A IFI16: Interferon-gamma-induced protein 16 NCOR/SMRT: Nuclear receptor corepressors COMT: Catechol-O-methyltransferase NK: Natural killer NKG2D: Natural killer group 2, member D CTCL: Cutaneous T-cell lymphoma PTCL: Peripheral T-cell lymphoma HAT: Histone acetyltransferase SDH: Succinate dehydrogenase TCA: Tricarboxylic acid cycle KGDHC: -Ketoglutarate dehydrogenase complex KAT2A/GCN5: Lysine acetyl-coenzyme A transferase 2A H3K79: Histone 3 lysine 79 -KG: Alpha-ketoglutarate CPT1A: Carnitine palmitoyltransferase 1A LDHA: Lactate dehydrogenase A K47: Lysine residue 47 H3K122: Histone 3 lysine 122 PGAM1: Phosphoglycerate mutase 1 GLS: Glutaminase SHMT2: Serine hydroxymethyltransferase 2 SDHA: Succinate dehydrogenase complex subunit A PKM2: Pyruvate kinase M2 PRMT5: Protein arginine methyltransferase 5 G6P: Glucose-6-phosphate CoAG6P: Coenzyme A glucose-6-phosphate SUCLA2VDAC3: Succinate-CoA ligase ADP-forming beta Subunit-voltage-dependent anion channel 3 SREBP-1CoA: Sterol regulatory element-binding protein-1 coenzyme A Fbw7 (FBXW7)SUCLA2: F-box and WD repeat-domain-containing 7 succinate-CoA Llgase ADP-forming beta subunit PGC-1SREBP-1: Peroxisome proliferator-activated receptor-gamma activator 1 alphasterol regulatory-element-binding protein-1 NF2Fbw7 (FBXW7): Neurofibromatosis type 2F-box and WD repeat-domain-containing 7 NMTsPGC-1: N-myristoyltransferase peroxisome-proliferator-activated Receptor-gamma coactivator 1 alpha PI3KNF2: Phosphoinositide 3-kinaseneurofibromatosis type 2 GAS41NMTs: Glioma-amplified sequence 41N-myristoyltransferases TNF-PI3K: Tumor necrosis factor-alpha phosphoinositide 3-kinase NDPGAS41: N-myristolytic downregulated protein glioma-amplified sequence 41 NUPTNF-: N-myristolytic upregulated protein tumor necrosis factor-alpha mTORNDP: Mammalian target of rapamycin N myristolytic downregulated protein AMLNUP: Acute myeloid leukemia N-myristolytic upregulated protein ICAM-1mTOR: Intracellular adhesion molecule 1 mammalian target of rapamycin FBXO4AML: F-box protein 4 acute myeloid leukemia PPT-1/2ICAM-1: Palmitoyl protein thioesterase 1/2 intracellular adhesion molecule 1 ABHD10/17FBXO4: / Hydrolase-domain-containing protein 10/17 F-box protein 4 FASNPPT-1/2: Fatty acid synthase palmitoyl protein thioesterase-1/2 EGFRABHD10/17: Growth factor receptor / hydrolase-domain-containing protein 10/17 PD-L1FASN: Programmed death ligand 1fatty acid synthase ACOX1EGFR: Acyl-coenzyme A oxidase 1epidermal growth factor receptor GLUT1PD-L1: Glucose transporter 1 programmed death ligand 1 NRasACOX1: Neuroblastoma RAS viral oncogene homologacyl-coenzyme A oxidase 1 GARS1GLUT1: Glycyl-tRNA synthetase 1glucose transporter 1 EVsNRas: Extracellular vesicle neuroblastoma RAS viral oncogene homolog CKAP4GARS1: Cytoskeleton-associated protein 4glycyl-tRNA synthetase 1 CD44EVs: Cluster of differentiation 44 extracellular vesicles STINGCKAP4: Stimulator of interferon gene cytoskeleton-associated protein 4 VDAC2CD44: Voltage-dependent anion channel 2 cluster of differentiation 44 PCSK9STING: Proprotein convertase subtilisin/kexin type 9 stimulator of interferon genes PTENVDAC2: Phosphatase and tensin homolog voltage-dependent anion channel 2 TeadsPCSK9: TEA domain transcription factor proprotein convertase subtilisin/kexin type 9 Tip60PTEN: TAT-interacting protein 60 phosphatase and tensin homolog SRCTeads: Steroid receptor coactivator TEA domain transcription factors ACAT1Tip60: Acetyl-CoA acetyltransferase 1TAT-interacting protein 60 CPT1ASRC: Carnitine palmitoyltransferase 1 asteroid receptor coactivator PATsACAT1: Protein acyltransferases acetyl-CoA acetyltransferase 1 ShhCPT1A: Sonic hedgehog carnitine palmitoyltransferase 1A HhatPATs: Hedgehog acyltransferase protein acyltransferases APTShh: Acyl protein thioesterase sonic hedgehog PPTHhat: Palmitoyl protein thioesterase hedgehog acyltransferase ABHD17A/B/CAPT: / Hydrolase-domain-containing protein 17A/B/cacyl protein thioesterase YEATSPPT: Yaf9, ENL, AF9, Taf14, and Sas5palmitoyl protein thioesterase BETABHD17A/B/C: Bromodomain and extraterminal / hydrolase-domain-containing protein 17A/B/C 2-BPYEATS: 2-Bromohexadecanoic acidYaf9, ENL, AF9, Taf14, and Sas5 BET: Bromodomain and extraterminal 2-BP: 2-Bromohexadecanoic acid References Bukiya AN, Dopico AM
Immunofluorescent stainings For staining of tissue sections, human and murine lung tissue was fixed in 4% formalin prior to paraffin embedding
