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Non-enzymatic glutathionylation of apocarotenoids and 4-HNE 12 nmol apocarotenoids were dried from ethanolic stocks, redissolved in 10 L DMSO and mixed with 190 L assay buffer to yield the following final concentrations in 200 L final volume: 60 M apocarotenoid and 510 mM GSH in 50 mM Tris (pH 9.0) containing 5% (v/v) DMSO