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PLoS One 6 , e18583 (2011)

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Stage-tips were activated with MeOH (2 60 L), washed with Buffer B (80% CH 3 CN, 20% water, 0.1% formic acid) (1 60 L), and equilibrated with Buffer A (2 60 L)

This limitation makes conventional vitamin C a poor candidate for therapeutic conditions that demand high absorption vitamin C for sustained or elevated plasma concentrations, such as immune modulation, oxidative stress recovery, and surgical healing