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Real-time qPCR was performed using an Applied Biosystems QuantStudio 12 K Flex real time PCR system (Thermo Fisher Scientific, San Jose, CA, USA) with THUNDERBIRD SYBR qPCR Mix (Toyobo), 6 pmol of primers, and aliquots of cDNA

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Most notably, large-scale clinical trials in the target population are lacking

The movement of fluorescent endocytic tracers in Plasmodium falciparum infected erythrocytes

The plates were incubated at 37 C with orbital shaking (180 rpm) between each read