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Cells were next washed with cold PBS0.1% Triton X-100 and incubated with goat anti-rabbit IgG coupled to Alexa Fluor 488 (1:1000) ( ex : 488 nm, em : 525/50 nm, Life technologies) in the PBS solution described above for 1 h at room in dark

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This should be avoided and tests should be delayed by 7-10 days post-injection

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