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Brudvik KW, Mise Y, Chung MH, Chun YS, Kopetz SE , Passot G, Conrad C, Maru DM, Aloia TA, Vauthey JN

The specific mechanisms by which oxidative stress favors the development of IPF are not yet fully understood although they are likely to target both the cell phenotype and life-cycle promoting aging and apoptosis

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To perform injections into lymph nodes, the lymphatics were first traced by injecting 2% Evans Blue dye (Sigma-Aldrich, E2129) into the foot pedal 5 min before performing intranodal injections

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ROS detection The mouse sperm was collected in the same way for each group of mice, and 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) from Reactive Oxygen Species (ROS) Assay Kit (Beyotime, Shanghai, China) was added to the samples