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The choice of site can depend on patient preference, muscle mass, and convenience

Animals subjected to in vivo microdialysis had another guide cannula (SciPro Scientific Products & Equipment, Ontario, Canada) inserted into the following coordinates (AP + 2.2 mm, LM + 1.2 mm, DV 5.6 mm from bregma) to measure extracellular dopamine level in the core region of nucleus accumbens

Now, invert the vial and draw your desired amount of bacteriostatic water (e.g., exactly 2mL) into the syringe

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