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L-glycine is the simplest naturally occurring amino acid from a structural standpoint, yet it plays a fundamental role in numerous physiological processes

Previous studies also demonstrated that ACNs from maize counteracted liver ROS directly, increased superoxidase dismutase 1 (SOD-1) and ameliorated liver damage, indicating that the observed reduction in oxidative stress can be attributed to the ROS scavenging activity of cyanidin-3-O-glucoside (Magaa-Cerino et al., 2020)

That said, if your fatigue stems from chronic toxin exposure or severe oxidative stress, you might notice more gradual improvement with repeated treatments

Anathy V, Aesif SW, Guala AS, Havermans M, Reynaert NL, Ho YS et al

Finally, the process demands careful balancing between maximizing extraction efficiency and preventing compound degradation, which remains a key limitation in fully harnessing pressurized liquid extraction for polyphenol extraction (77)