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Then cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kits (4374967, Applied Biosystems) with the accompanied random primers, as described previously ( The mRNA expression levels of given genes in the transfected cells with or without cell sorting enrichment were determined by qPCR using Taq388 mix (Supplementary Table S3

All RNA was aliquoted and stored at 80 C

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ADCC ADCC was performed by an [ 51 Cr] release assay 50