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Without M6P modification, luminal enzymes, represented by CTSC and CTSD, cannot traffic to lysosomes and mature, resulting in a significant fraction being secreted into the media as unprocessed proenzymes (Fig

We used our established genetic model in TrC1 prostate cancer cells expressing either AKT1-WT or the clinically relevant activation associated AKT1-E17K mutant and pharmacologic approaches to explore how cancer cell-specific aberrant AKT activation and the activity of AKT-regulated metabolic pathways impact metabolic state and antioxidant defense at the basal state as well as changes in cellular levels of GSH, NADPH, and ROS, short-term and long-term survival upon irradiation

It is a modular structure composed of a zinc finger protein DNA-binding domain fused to the FokI restriction endonuclease cleavage domain

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Surprisingly, it has been found that this fragment retained practically all effects of the natural BPC protein (EP 0572688 and U.S