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Also Cpt1b m/ and Cpt1b fl/fl mice were fed a high fat-diet consisting of 45% calories from fat (D12451, Research Diets Inc, New Brunswick, NJ, USA) starting at 8 week of age for 16 weeks prior to the experiments, whereas mice from low fat-diet group were fed a chow diet consisting of 10% calories from fat (D12450, Research Diets Inc, New Brunswick, NJ, USA) starting at weaning age until they were utilized for experiments