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Description

The oven program consisted of an initial hold at 100C for 1 min, a ramp to 160C at 10C/min, a ramp to 200C at 5C/min, and a final ramp to 330C at 10C/min with a 4 min hold

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Protein disulfide-isomerase, a folding catalyst and a redox-regulated chaperone

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Tsutsumi, K

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Transfer the uppermost supernatant to an EP tube containing 500 l of isopropanol, mix well, and let it stand for 10 min

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Furthermore, moisture and humidity can accelerate hydrolysis, breaking down peptide bonds and leading to fragmentation

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10.1016/j.toxicon.2021.06.005 19

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