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To investigate the effect of SeNPs on psoriasis in vivo, IMQ was used to induce psoriasis which is a widely accepted animal model, acts by binding to Toll-like receptors (TLR7 and TLR8), which involved in activation of innate immunity and stimulates adaptive immunity and recapitulates typical histopathological features in mice such as parakeratosis, acanthosis, scaling, erythema, skin thickening, and inflammatory cell infiltration which closely resembles human psoriasis [76]

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The intracellular metabolite extracts and 2 mL of spent culture medium containing extracellular metabolites were freeze-dried (BenchTop K manifold freeze dryer, VirTis) prior chemical derivatisation

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Carlson CG, Huang DT