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An SD diet exacerbates weight loss, reduces colon length, and increases stool bleeding and diarrhea in models of DSS-induced colitis and in shPsat1 mice (short hairpin RNAs, shRNAs targeting the 2nd gene in the SSP pathway), consistent with worsened disease severity and inflammation 62,114

Currently, the widely utilized animal models of MDD include CUMS, behavioral despair (BD), learned helplessness (LH), and CSDS, drug withdrawal, and transgenic animal models

Several studies identified DNA-sensing pattern recognition receptors (PRRs) as drivers of IFN I-driven inflammation and massive cell death in severe COVID-19: cyclic GMPAMP synthase (cGAS), absent in melanoma (AIM)2, nucleotide-binding oligomerization domain (NOD)-like leucine-rich repeat (LRR) and PYRIN domain containing (NLRP)3, receptor for advanced glycation end products (RAGE), and Toll-like receptor (TLR)9 (9, 13, 14, 1723): Upon detecting double stranded (ds)DNA, these PRRs activate the production of antiviral and pro-inflammatory cytokines and chemokines and eventually, cell death, via two principal pathways (reviewed in (24, 25))

10.1097/MAO.0b013e3182267e06 32 KapoorN.ShyamR.SinghA.ManiK.SharmaR.SelvamurthyW

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2.3) Characterization of cysteine-conjugated antibody-drug conjugates The resulting conjugates were characterized as follows: Drug-Antibody-Ratio (DAR) assessment by reverse phase liquid chromatography-mass spectrometry (RPLC-MS): Denaturing RPLC-QToF analysis was performed using the HPLC method 4 described above in Example 1