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Cells were then washed twice in NB medium, counted, resuspended in NB media [Neurobasal (NB) medium: Neurobasal (21103049, Life Technologies), 1 N2 supplement, 1 B27 supplement, 1 Glutamax, 1 NEAA, 1 penicillin-streptomycin, 3.2 mg/ml glucose and 0.2 mM ascorbic acid]

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[DOI] [PMC free article] [PubMed] [Google Scholar] 147.Altamirano J, Bataller R

2013;3:e309

Rice-derived porous carbon, prepared through expansion and nitrogen doping, was used as the positive electrode in ZIHSCs 57

The use of defined novel skeleton structures whose synthesis can be scaled up in preparation for hit validation, along with optimized downstream reactions with commercially available building blocks, will facilitate the process of hit validation