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Amplification and real-time analysis were performed on a Step One Plus System (Applied Biosystems, Waltham, MA, USA)

Screens were analyzed using Python (v.2.7.13), R (v.3.3.2) and Unix (v.4.10.0-37-generic x86_64)

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The precise mechanism underlying ferroptosis is still unknown, but it is known that altered iron metabolism, glutathione (GSH) depletion, GPX4 inactivation, and increased PUFA peroxidation by ROS play key roles in its onset and progression [80, 81]

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19 These controversial observations call for further mechanistic studies on mitochondria and energy metabolism in different cell types