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Samples were eluted using 30uL (at 1mg/mL) of synthetic SUMO2/3 peptide (Amino Acid Sequence: IRFRFDGQPI, synthesized through Genscript) per sample and incubating at 37 C for 15 minutes at 900rpm on a thermomixer

We predict that breast cancer cells treated with ATZ alone should have a greater proliferation rate associated with stronger EGF signaling activity, while the co-treatment of ATZ+antagonist and ATZ+Curcumin should produce limited proliferation and diminished EGF signaling activity

Long-term effects, even in animals, have not been studied

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Spi 8, 450457

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