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LENTIVIRAL TRANSDUCTION: B16 parental and GOT1/2 dKO knockout cells were thawed from liquid nitrogen stocks, recovered in culture for 12 days, trypsinized and seeded at 2.511101cells per well in 21mL of complete RPMI medium (10% FBS, P/S, 1 mM sodium pyruvate and 204 M uridine) in 6-well plates (8 wells per cell type) to achieve 100x coverage per sgRNA

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[DOI] [PMC free article] [PubMed] [Google Scholar] 158.Fardsadegh B., Vaghari H., Mohammad-Jafari R., Najian Y., Jafarizadeh-Malmiri H

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