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The purified GST- jr PPO1-wt fusion protein was exchanged to a glutathione-free buffer containing 200 mM NaCl and 50 mM Tris-HCl at pH 7.8 and 1 mM dithiothreitol (DTT) using a Vivaspin ultrafiltration device (VWR, molecular weight cut-off 30 kDa)

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The food contains 39.13% protein, 15.21% fat, 12.63% fiber, and 345 kcal per can or 25.5 kcal per ounce

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