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Separation procedure in affinity chromatography can be simply illustrated as shown in Figure 1

55 This was not a region that Pirh2 reportedly bound to on p53 (Figure 1), suggesting that p28 did not directly block the binding of Pirh2 on p73 and alter its ubiquitination

-glucan is a kind of polysaccharide that typically has a glucose backbone connected by -(1,3)-glycosidic bonds and maybe extra glucose residues linked by -(1,6) connections