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A Phase Ib Study of JS001, a Humanized IgG4 mAb Against Programmed Death-1 (PD-1) Combination With Axitinib in Patients With Metastatic Mucosal Melanoma

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Approximately 50 mg of cerebral cortex tissue was homogenized in RIPA lysis buffer supplemented with a protease inhibitor cocktail (Santa Cruz Biotechnology, sc-24948, USA), followed by centrifugation at 16,000 g for 20 min at + 4 C to obtain the supernatants