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Immunoprecipitation Cells were harvested 36 h post-transfection and then lysed in radioimmunoprecipitation assay (RIPA) lysis buffer (50 mM Tris-HCl, 150 mM NaCl, 0.5% sodium deoxycholate, 1% IGEPAL, 1 mM NaF, 1 mM Na3VO4) containing a protease inhibitor cocktail and a phosphatase inhibitor cocktail (Sigma)

doi: 10.1016/j.jbc.2021.101365 70 GnanapradeepanKBasuSBarnoudTBudina-KolometsAKungCPMurphyME

Covalent insertion of antioxidant molecules on chitosan by a free radical grafting procedure

Additionally, animal studies such as the one by Gurger et al

Currently, a few cationic liposome-based nanocarriers have been designed for nonviral gene delivery to retinal tissues