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Step 6: Verify the solution Hold the reconstituted vial up to light (briefly, to minimize light exposure)

[PubMed] [Google Scholar] 66.Cai J, Huang ZZ, Lu SC

Nature Reviews Endocrinology .

The copy numbers of integrated genes were verified using real-time PCR (primers GshF-RT1/GshF-RT2, Additional file 1: Table S7) and calculated by a comparative C T method [27]