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Biochem Biophys Res Commun 363:921925 Wu P, Dong J, Cheng N, Yang R, Han Y, Han Y (2019) Inflammatory cytokines expression in Wilsons disease

Cells were treated with ENDOs or vehicle and TLR9 activation in response to CpG (5 M) was analyzed by measuring SEAP activity, monitored using QUANTI-Blue (InvivoGene) following the manufacturers recommendations

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Cellular senescence in cancer: from mechanisms to detection

The membranes were then incubated with the appropriate secondary antibodies (Santa Cruz Biotechnology, Inc.), conjugated with horseradish peroxidase (HRP) for 2 h at room temperature and rinsed three times with PBS