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For ACA treatment in -catenin-driven mouse liver tumor models, 6-8 weeks male C57BL/6 N mice were transfected with c-Met/-catenin plasmids (2 mL of saline containing 20 g pT3-EF1H c-Met plasmid, 20 g pT3-EF1H N90-beta-catenin plasmid, and 1.6 g pCMV/sleeping beauty transposase plasmid) or AKT/-catenin plasmids (2 mL of saline containing 20 g pT3-EF1H myr-AKT-HA plasmid, 20 g pT3-EF1H N90-beta-catenin plasmid, and 1.6 g pCMV/sleeping beauty transposase plasmid) via hydrodynamic tail vein injection within 5 to 7 seconds

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