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Beyond its role as a direct antioxidant, glutathione is the primary agent in Phase II liver detoxification, a critical process for clearing harmful substances from the body

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Notably, the mRNA increase of all the genes evaluated was abolished by the use of a neutralizing anti-NGF antibody (Figure 5)

For each reaction, 100 g of purified enzyme (diluted in 10 mM base buffer) in a total volume of 50 l is incubated with equal volume of substrate-liposome mix (prepared by mixing of 30 l 10 mM Dioleoylphosphatidylcholine, 30 l 10 mM Dioleoylphosphatidylglycerol, and 30 l 1 mM PLA 2 substrate) for 10 min at room temperature