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In this experimental platform, the anti-gravity calf-muscle group (gastrocnemius (Gas), plantaris (Pla), and soleus (Sol) muscles) and shank muscles (tibialis anterior (TA) and extensor digitorum longus (EDL)) were collected from four experimental groups of 8-week-old C57BL/6J male mice: i) control (without any treatment), ii) 5-days suspension-disuse, iii) 3-days LASSS pre-administration (about 50 g in drinking water/g body weight/day) followed by 5-days suspension-disuse treatment without LASSS supplementation, and iv) 3-days GSSSG pre-administration followed by suspension-disuse treatment ( n = 3 mice per group)

Chromatogram results were used for the formation of calibration curves for the measured analytes

Cloning and recombination of plasmids and fragments were performed using the One Step Cloning Kit provided by Vazyme (#C112, Nanjing, Jiangsu, China)

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Anyone experiencing low energy, high stress, poor immune function, or a diet lacking in B vitamins may benefit

In an in vitro study, yakuchinone A isolated from the extract could reduce the production of IL-17 and inhibit the EAE symptoms