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For decades, tobacco companies have tried to develop alternative products to replace dwindling sales of cigarettes, as smoking levels in the U.S

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For cardosin B processing assay, concentrated fractions were diluted 1:1 in phosphate buffer 100 mM (pH 7 and 6) or citrate buffer 100 mM (pH 5, 4 and 3) and incubated at 37 C, samples were collected and analyzed by western blot

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