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"Biomolecular archaeology reveals ancient origins of indigenous tobacco smoking in North American Plateau" (PDF)

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After measuring protein concentrations using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific), the homogenates (20 g total protein) were separated on 12% SDSPAGE gels and transferred to polyvinylidene fluoride membranes

Theres nothing worse for me than having too much of a good thing, whether it be a nicotine buzz or a dominant flavor

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