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There are several approaches to testing metabolomics: Targeted metabolomics Hypothesis testing strategy Low number of metabolites studied Identity of metabolites is known a priori Absolute quantification of metabolites Untargeted metabolomics Hypothesis generating/discovery strategy High number of metabolites studied (typically 100-1000s) Identity of metabolites are unknown a priori Relative quantification of metabolites To better understand the metabolic landscape and potential health consequences of e-cigarettes, Dr