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To confirm that PPP activity is required for CR formation, cells were treated with 6-aminonicotinamide (6-AN), a competitive inhibitor of 3-phosphoglycerate dehydrogenase (PHGDH), which catalyzes the rate-limiting step of the PPP, and the levels of CR were monitored by LC-MS/MS

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But different mechanisms for delivery smoking, vaping, edibles, skincare products, coffees only complicate the issue

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