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For the experimental procedures, Caco-2 cells were subcultured and utilized at passages 510 in DMEM containing 10% FBS and 1% penicillin/streptomycin and incubated at 37 C in a humidified atmosphere with 5% CO

Worldwide, smoking killed more than 100 million people during the 20th Century, according to the World Health Association

The chest cavity is found just above the abdominal cavity, and the two are separated by the diaphragm, a muscular and membranous partition