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RT-qPCR The total RNA of cultured epithelial cell lines was extracted by using TRIzol (Invitrogen, CA, USA) reagent according to the manufacturers protocol

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In Shakira's music video "Objection (Tango)", when Shakira is about to fight with her boyfriend's new lover after fantasizing to defeat her rival single-handedly in an animated sequence, Shakira is strained by her boyfriend and said woman blows a cigar smoke onto Shakira's face

The episode, part of The X-Files excellent fourth season, is called Musings Of A Cigarette Smoking Man, and it opens with the eponymous villainthe mysterious, influential government operative known only at the time as either that or The Cancer Mansetting up surveillance on Mulder, Scully, and Mulders trio of conspiracy theorist buddies The Lone Gunmen

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