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Insulin sensitivity was evaluated by calculating the quantitative insulin sensitivity check index (QUICKI) 30 , and glomerular filtration rate (GFR) was estimated using the CKD-EPI creatinine-cystatin C equation 31

showed that the extract from electronic cigarettes (containing different flavours and nicotine quantity) applied to cultured myocardial cells at different dilution, both with or without nicotine, was cytotoxic at different concentrations irrespective of nicotine presence

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