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Taffeta scripts ( were used to analyze the RNA-Seq data, which included trimming of adapters using trimmomatic (v.0.32) 54 and using FastQC 55 (v.0.11.2) to obtain overall QC metrics

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[171] As part of this franchise agreement, 7-Elevens in Oklahoma bore slight differences to stores elsewhere: for instance, products such as Big Bite hot dogs were not sold there, the Slurpee was branded as the "Icy Drink", and Oklahoma stores operated their own loyalty program called "Thx!", which did not intersect with the national 7Rewards system

In fact, some of the same actors responsible for manufacturing tobacco products that have killed upwards of 20 million Americans have jumped at the opportunity to get on the e-cigarette bandwagon

In the baseline scenario, age-specific initiation and cessation rates were assumed to remain constant in all years, with no new product initiation (and therefore no new switching between products or new dual use) after the age of 30 years