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To determine whether GSH depletion can also exert protective effects in a primarily MASH model, we overexpressed Chac1 in mouse liver using AAV8 followed by challenging mice with methionine/choline-deficient diets (MCDs) to induce MASH

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While 0 generally indicated no changes, the lesions were subsequently scored as follows: 0-3, edema (1- weak diffuse and/or perifocal