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This agreement provided the basis for the current debate in Congress over national tobacco legislation

15 of 2009 on Combating Tobacco (2009)

Intramolecular circularization of the DNA was performed with T4 DNA ligase (NEB) and residual linear DNA was degraded by a cocktail of exonucleases containing plasmid-safe ATP-dependent DNase (Lucigen), lambda exonuclease (NEB) and exonuclease I (NEB)