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The An1b protein coding region was amplified by PCR using An1b-F and An1b-R (primer sequences are listed in Supplementary Table S2) and cloned into the pGADT7 vector using the ClonExpress II One Step Cloning Kit (Vazyme, China) to generate pGADT7-An1b

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On top of that, they create hazardous waste chemicals still present in what people throw away, Vandenbroucke said

What makes human milk oligosaccharides unique are the specific set of linkages, or rules, for connecting simple sugars together that are found in these molecules