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Increased use of e-cigarettes by youth has raised concerns about the trying out of e-cigarettes and then taking up cigarette smoking, often referred to as the gateway to smoking 9,10

Taken together with the direct measurements of transporter protein expression (Fig 2F and 2G), our results in Fig 2 strongly implicate KCC2 impairment following nicotine exposure

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The use of smokeless tobacco is seen primarily in men and adolescent boys who are rural residents of southern and western states

The expression of several other proteins is induced in a similar manner