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Cryo-EM sample preparation and data acquisition Four microliters of the sample (protein concentration: 3 mg/mL, liposome concentration: 1.5 mg/mL) were loaded onto a Quantifoil Au grid (R2/2, 300 mesh), followed by plunge-freezing in liquid ethane using a Vitrobot Mark IV (Thermo Fisher) at 4 C and 100% humidity

The most harmful components of cigarette smoke include tar

Sajid M, Srivastava S, Yadav RK, Singh H, Singh S, Bharadwaj M (2022) Composition and ecological functionality of fungal communities associated with smokeless tobacco products mainly consumed in India

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Though smaller than cigarettes or vaping items, chewing tobacco remains significant as a conventional smokeless commodity within Germany