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"She wasn't just one of those rich women who said, 'Restore me 60 houses.' " Though she still would occasionally joke, "I haven't had any greens lately," which Nancy Cooke de Herrera says was Duke's code for sex, she seemed to be settling down

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After tissue fixation and paraffin embedding, 5 m sections were incubated with anti-E-cadherin, ZO-1, Keap1 and Nrf2 antibodies (1:100 dilution) at 4 overnight followed by incubation with secondary antibody, at last stained with DAB and counterstained with hematoxylin