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In contrast, the application of 250 L (221 L/cm 2 , equivalent to the addition of 73.5 L to a 6.5 mm cell culture insert) was the smallest volume tested that maintained complete coverage across the insert, while also exhibiting better uniformity than the 200 L volume, by 24 h

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But it would end up being too hard, and ultimately everything would dissolve

It is reminded businesses, or members of the public, that if they have any information regarding smuggling, they can contact Revenue in confidence on Confidential Phone Number 1800 295 295