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Name: does john travolta smoke cigarettes SATURDAY NIGHT FEVER"-ORIGINAL PHOTO-JOHN TRAVOLTA-PORTRAIT-SMOKING We never get tired of
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The thermal cycles included an initial step at 95 C for 3 min, followed by 25 cycles at 95 C, 55 C, and 72 C for 30 s each, and a final cycle at 72 C for 5 min