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Cigarette smoke extract (CSE) was prepared fresh (used within 1 hr) from filter-less research-grade cigarettes (3R4F, Tobacco Research Institute, University of Kentucky, Lexington, KY) as described previously [5]

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They start smoking daily sooner and progress more rapidly from first cigarette to daily smoking

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[2] [3] [4] The C-ration differs from other American alphabetized rations such as the A-ration, consisting of fresh food