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The amplification was performed using Illumina Nextera XT adapter-ligated eubacterial V3 region-specific primers, 341F and 534R, with five different base modifications ( The underlined regions in all the primer sequences are the Illumina Nextera XT adapter overhangs, whereas the non-underlined regions are the primer sequences known to target V3 region of eubacterial 16S rRNA gene

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Following the now-famous twist at the end of the 2016 pilot episode of NBCs family drama This Is Us, its hard to remember a time when the world didnt know the show was not about separate people in different circumstances all living in modern day, but a time-jumping show about one family over the span of decades

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